NT5E monoclonal antibody, clone AT89E9
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HepG2 cell lysate, Lane 2: WiDr cell lysate, Lane 3: A431 cell lysate.
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with NT5E monoclonal antibody, clone AT89E9 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry
Flow cytometric analysis of A-431 cells. The cell was stained with NT5E monoclonal antibody, clone AT89E9 at 2-5 ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant NT5E.
Immunogen
Recombinant protein corresponding to amino acids 27-252 of human NT5E.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HepG2 cell lysate, Lane 2: WiDr cell lysate, Lane 3: A431 cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with NT5E monoclonal antibody, clone AT89E9 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of A-431 cells. The cell was stained with NT5E monoclonal antibody, clone AT89E9 at 2-5 ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray). -
Gene Info — NT5E
Entrez GeneID
4907GeneBank Accession#
NM_002526Protein Accession#
NP_002517Gene Name
NT5E
Gene Alias
CD73, E5NT, NT, NT5, NTE, eN, eNT
Gene Description
5'-nucleotidase, ecto (CD73)
Omim ID
129190Gene Ontology
HyperlinkGene Summary
Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM
Other Designations
5' nucleotidase (CD73)|5' nucleotidase, ecto|OTTHUMP00000016808|OTTHUMP00000040565|Purine 5-Prime-Nucleotidase|ecto-5'-nucleotidase
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Interactome
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Pathway
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Disease
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Publication Reference
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Physiological roles for ecto-5'-nucleotidase (CD73).
Colgan SP, Eltzschig HK, Eckle T, Thompson LF.
Purinergic Signalling 2006 Jun; 2(2):351.
Application：Flow Cyt, Mouse , Mouse smooth muscle cells.
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Crucial role for ecto-5'-nucleotidase (CD73) in vascular leakage during hypoxia.
Thompson LF, Eltzschig HK, Ibla JC, Van De Wiele CJ, Resta R, Morote-Garcia JC, Colgan SP.
The Journal of Experimental Medicine 2004 Dec; 200(11):1395.
Application：Flow Cyt, Mouse, Mouse leukocytes.
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Differential regulation and function of CD73, a glycosyl-phosphatidylinositol-linked 70-kD adhesion molecule, on lymphocytes and endothelial cells.
Airas L, Niemela J, Salmi M, Puurunen T, Smith DJ, Jalkanen S.
The Journal of Cell Biology 1997 Jan; 136(2):421.
Application：Dot, Flow Cyt, IF, IP, Human, HEC cells, HUVECs, Lymphocytes.
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Physiological roles for ecto-5'-nucleotidase (CD73).
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