BHMT monoclonal antibody, clone 3D6
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of mouse liver tissue lysate.
Immunofluorescence
Immunofluorescence analysis of Hep3B cells line, stained with DAPI (Blue) for nucleus staining and BHMT monoclonal antibody, clone 3D6 (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Flow Cytometry
Flow cytometric analysis of Hep3B cell line, staining at 2-5 ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant BHMT.
Immunogen
Recombinant protein corresponding to full length human BHMT.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Isotype
IgG2a, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse liver tissue lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of Hep3B cells line, stained with DAPI (Blue) for nucleus staining and BHMT monoclonal antibody, clone 3D6 (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Hep3B cell line, staining at 2-5 ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. -
Gene Info — BHMT
Entrez GeneID
635Protein Accession#
AAH12616Gene Name
BHMT
Gene Alias
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Gene Description
betaine-homocysteine methyltransferase
Omim ID
602888Gene Ontology
HyperlinkGene Summary
This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq
Other Designations
betaine homocysteine methyltransferase
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Interactome
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Pathway
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Disease
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