DAXX monoclonal antibody, clone 7A11
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using DAXX monoclonal antibody, clone 7A11 (Cat # MAB10621) against K-562 cell lysate.
Immunofluorescence
Confocal immunofluorescence analysis of HeLa cells using DAXX monoclonal antibody, clone 7A11 (Cat # MAB10621) (green) . Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence
Confocal immunofluorescence analysis of PANC-1 cells using DAXX monoclonal antibody, clone 7A11 (Cat # MAB10621) (green) . Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry
Flow cytometric analysis of HeLa cells using DAXX monoclonal antibody, clone 7A11 (Cat # MAB10621) (green) and negative control (purple).
-
Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant DAXX.
Immunogen
Recombinant protein corresponding to human DAXX.
Host
Mouse
Theoretical MW (kDa)
81
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis using DAXX monoclonal antibody, clone 7A11 (Cat # MAB10621) against K-562 cell lysate.Immunofluorescence
Confocal immunofluorescence analysis of HeLa cells using DAXX monoclonal antibody, clone 7A11 (Cat # MAB10621) (green) . Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Immunofluorescence
Confocal immunofluorescence analysis of PANC-1 cells using DAXX monoclonal antibody, clone 7A11 (Cat # MAB10621) (green) . Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells using DAXX monoclonal antibody, clone 7A11 (Cat # MAB10621) (green) and negative control (purple). -
Gene Info — DAXX
Entrez GeneID
1616Gene Name
DAXX
Gene Alias
BING2, DAP6, EAP1, MGC126245, MGC126246
Gene Description
death-domain associated protein
Omim ID
603186Gene Ontology
HyperlinkGene Summary
This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
CENP-C binding protein|ETS1-associated protein 1|Fas-binding protein|OTTHUMP00000029289|OTTHUMP00000029290|death-associated protein 6
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com