RELA monoclonal antibody, clone 27F9.G4
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HeLa cells with RELA monoclonal antibody, clone 27F9.G4 (Cat # MAB10259) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed paraffin-embedded human colon carcinoma with RELA monoclonal antibody, clone 27F9.G4 (Cat # MAB10259).
Immunofluorescence
Immunofluorescence staining of HeLa cells with RELA monoclonal antibody, clone 27F9.G4 (Cat # MAB10259) at 1:5000 dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of RELA.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human RELA.
Host
Mouse
Theoretical MW (kDa)
65
Reactivity
Human
Specificity
This product showed a single band by IEP (immunoelectrophoresis) when tested with anti-mouse antibody. Reactivity was confirmed by ELISA against peptide conjugated carrier protein and by Western blot against HeLa whole cell lysate.
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
IHC (1:200-1:600)
IF (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HeLa cells with RELA monoclonal antibody, clone 27F9.G4 (Cat # MAB10259) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed paraffin-embedded human colon carcinoma with RELA monoclonal antibody, clone 27F9.G4 (Cat # MAB10259).Immunofluorescence
Immunofluorescence staining of HeLa cells with RELA monoclonal antibody, clone 27F9.G4 (Cat # MAB10259) at 1:5000 dilution. -
Gene Info — RELA
Entrez GeneID
5970Gene Name
RELA
Gene Alias
MGC131774, NFKB3, p65
Gene Description
v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Omim ID
164014Gene Ontology
HyperlinkGene Summary
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM
Other Designations
nuclear factor of kappa light polypeptide gene enhancer in B-cells 3|v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (p65))|v-rel reticuloendotheliosis viral oncogene homolog
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Interactome
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Pathway
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Disease
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