CASQ2 monoclonal antibody, clone AT4E10
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of mouse muscle extracts (35 ug) with CASQ2 monoclonal antibody, clone AT4E10 (Cat # MAB10015) at 1:500 (Lane 1), 1:1000 (Lane 2) and , 1:5000 (Lane 3) dilution. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with CASQ2 monoclonal antibody, clone AT4E10 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CASQ2.
Immunogen
Recombinant protein corresponding to amino acids 20-399 of human CASQ2.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Concentration
1 mg/mL
Isotype
IgG1, kappa
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse muscle extracts (35 ug) with CASQ2 monoclonal antibody, clone AT4E10 (Cat # MAB10015) at 1:500 (Lane 1), 1:1000 (Lane 2) and , 1:5000 (Lane 3) dilution. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with CASQ2 monoclonal antibody, clone AT4E10 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — CASQ2
Entrez GeneID
845Protein Accession#
NP_001223Gene Name
CASQ2
Gene Alias
FLJ26321, FLJ93514, PDIB2
Gene Description
calsequestrin 2 (cardiac muscle)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene specifies the cardiac muscle family member of the calsequestrin family. Calsequestrin is localized to the sarcoplasmic reticulum in cardiac and slow skeletal muscle cells. The protein is a calcium binding protein that stores calcium for muscle function. Mutations in this gene cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest. [provided by RefSeq
Other Designations
OTTHUMP00000013749|calsequestrin 2, fast-twitch, cardiac muscle|cardiac calsequestrin 2
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Interactome
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Disease
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