TUBG1 monoclonal antibody, clone TU-30
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of TUBG1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human TUBG1.
Host
Mouse
Theoretical MW (kDa)
48
Reactivity
Chicken, Human, Mouse, Pig, Rat
Specificity
This antibody recognizes C-terminal peptide sequence EYHAATRPDYISWGTQ (aa 434-449) of gamma-tubulin, a 48 KDa structural constituent of cytoskeleton and microtubule organizing center (MTOC).
Form
Liquid
Isotype
IgG2b
Recommend Usage
Immunocytochemistry (1-2 ug/mL)
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunocytochemistry
Immunofluorescence staining of microtubular networks in NIH/3T3 mouse fibroblasts. A : Metaphase. B : Anaphase. C : Telophase. Gamma - tubulin (red) stained with TUBG1 monoclonal antibody, clone TU - 30 (Cat # MAB0975), alpha - tubulin (green) with polyclonal anti - alpha - tubulin antibody and nuclei with DAPI (blue). -
Gene Info — TUBG1
Entrez GeneID
7283Gene Name
TUBG1
Gene Alias
TUBG, TUBGCP1
Gene Description
tubulin, gamma 1
Omim ID
191135Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. [provided by RefSeq
Other Designations
tubulin, gamma polypeptide
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Interactome
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Disease
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