His tag monoclonal antibody, clone H21-5
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant protein with His tag.
Immunogen
Polypeptide containing the N-terminus 6 x His tag.
Host
Mouse
Specificity
This antibody has been selected for its ability to detect proteins containing consecutive histidine regions. The antibody detects polyhistidine tags localized at the N-terminus.
Form
Lyophilized
Preparation Method
This antibody was produced from a mouse hybridoma (clone# H21-5) elicited from a mouse immunized with a polypeptide containing the N-terminus 6 x histidine tag. The lgG fraction of ascites fluid was purified by Protein G affinity chromatography.
Isotype
IgG3, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein with His tag on ELISA and Western Blot and Affinity chromatography.
Recommend Usage
Affinity chromatography
ELISA
Western Blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS.
Storage Instruction
Store at -20°C on dry atmosphere.
Keep the vial on ice prior to restoration. For preparing a 1 mg/mL stock solution, reconstitute the antibody by addition of 100 uL of 50% glycerol, mix gently and let it stand for 5 min on ice. Using deionized water instead of 50% glycerol may be good, but for extended storage, 50% glycerol is recommended. For an optimal storage, aliquot into small portions and store at -20°C or below. Avoid repeated freeze-thaw cycles. -
Applications
Western Blot (Cell lysate)
Western Blot (Recombinant protein)
Enzyme-linked Immunoabsorbent Assay
Affinity Chromatography
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