MBS/MYPT1 (phospho T696) monoclonal antibody, clone AF-20
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Enzyme-linked Immunoabsorbent Assay
ELISA for measurement of recombinant Rho-kinase activity using MBS/MYPT1 (phospho T696) monoclonal antibody, clone AF-20 (Cat # MAB0001).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic phosphopeptide of MBS/MYPT1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T696 of human MBS/MYPT1.
Host
Mouse
Theoretical MW (kDa)
135
Reactivity
Chicken, Human, Mouse, Rat
Specificity
Phospho-MBS/MYPT1 Thr696 Antibody detects endogenous MBS/MYPT1 only when phosphorylated at threonine696. The antibody does not recognize other myosin phosphatase regulatory subunit.
Form
Liquid
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Western Blot (1-2 ug/mL)
ELISA (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM phosphate buffer, 300 mM NaCl, pH 7.5 (50% glycerol).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
ELISA for measurement of recombinant Rho-kinase activity using MBS/MYPT1 (phospho T696) monoclonal antibody, clone AF-20 (Cat # MAB0001). -
Gene Info — PPP1R12A
Entrez GeneID
4659GeneBank Accession#
D87930Protein Accession#
BAA22378Gene Name
PPP1R12A
Gene Alias
MBS, MGC133042, MYPT1
Gene Description
protein phosphatase 1, regulatory (inhibitor) subunit 12A
Omim ID
602021Gene Ontology
HyperlinkGene Summary
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
myosin phosphatase, target subunit 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Microglial ROCK is essential for chronic methylmercury-induced neurodegeneration.
Shinozaki Y, Danjo Y, Koizumi S.
Journal of Neurochemistry 2019 Jul; [Epub].
Application：IF, IHC, WB, Mouse, Mouse cortices, Mouse microglia.
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Characterisation of the Cullin-3 mutation that causes a severe form of familial hypertension and hyperkalaemia.
Schumacher FR, Siew K, Zhang J, Johnson C, Wood N, Cleary SE, Al Maskari RS, Ferryman JT, Hardege J, Yasmin, Figg NL, Enchev R, Knebel A, O'Shaughnessy KM, Kurz T.
EMBO Molecular Medicine 2015 Oct; 7(10):1285.
Application：WB, Mouse, Aortic vessel.
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Microglial ROCK is essential for chronic methylmercury-induced neurodegeneration.
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