MAP3K5 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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More Files
- More Functions
SDS-PAGE Gel
MAP3K5 transfected lysate
Western Blot
Lane 1: MAP3K5 transfected lysate ( 155 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-MAP3K5 full length
Host
Human
Theoretical MW (kDa)
155
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MAP3K5 antibody (H00004217-M03) by Western Blots.
SDS-PAGE Gel
MAP3K5 transfected lysate
Western Blot
Lane 1: MAP3K5 transfected lysate ( 155 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — MAP3K5
Entrez GeneID
4217GeneBank Accession#
BC054503Protein Accession#
AAH54503Gene Name
MAP3K5
Gene Alias
ASK1, MAPKKK5, MEKK5
Gene Description
mitogen-activated protein kinase kinase kinase 5
Omim ID
602448Gene Ontology
HyperlinkGene Summary
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq
Other Designations
MAP/ERK kinase kinase 5|MAPK/ERK kinase kinase 5|OTTHUMP00000017275|apoptosis signal regulating kinase
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Interactome
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Pathway
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