TIPIN HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-TIPIN full length
Host
Human
Theoretical MW (kDa)
34
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TIPIN antibody (H00054962-M01) by Western Blots.
SDS-PAGE Gel
FLJ20516 transfected lysate
Western Blot
Lane 1: FLJ20516 transfected lysate ( 34 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
-
Applications
Western Blot
Immunoprecipitation
-
Gene Info — TIPIN
-
Interactome
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com