GALT HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-GALT full length
Host
Human
Theoretical MW (kDa)
43
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GALT antibody (H00002592-M01) by Western Blots.
SDS-PAGE Gel
GALT transfected lysate
Western Blot
Lane 1: GALT transfected lysate ( 43 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — GALT
Entrez GeneID
2592GeneBank Accession#
BC015045Protein Accession#
AAH15045Gene Name
GALT
Gene Alias
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Gene Description
galactose-1-phosphate uridylyltransferase
Gene Ontology
HyperlinkGene Summary
Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. [provided by RefSeq
Other Designations
OTTHUMP00000021298|galactose-1-phosphate uridyl transferase
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Interactome
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Pathway
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Disease
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