FASN HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-FASN full length
Host
Human
Theoretical MW (kDa)
273
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-FASN antibody (H00002194-M02) by Western Blots.
SDS-PAGE Gel
FASN transfected lysate
Western Blot
Lane 1: FASN transfected lysate ( 273 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — FASN
Entrez GeneID
2194GeneBank Accession#
BC007909Protein Accession#
AAH07909Gene Name
FASN
Gene Alias
FAS, MGC14367, MGC15706, OA-519, SDR27X1
Gene Description
fatty acid synthase
Omim ID
600212Gene Ontology
HyperlinkGene Summary
The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq
Other Designations
short chain dehydrogenase/reductase family 27X, member 1
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