DNAJB4 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-DNAJB4 full length
Host
Human
Theoretical MW (kDa)
38
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DNAJB4 antibody (H00011080-M01) by Western Blots.
SDS-PAGE Gel
DNAJB4 transfected lysate
Western Blot
Lane 1: DNAJB4 transfected lysate ( 38 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — DNAJB4
Entrez GeneID
11080GeneBank Accession#
BC034721Protein Accession#
AAH34721Gene Name
DNAJB4
Gene Alias
DNAJW, DjB4, HLJ1
Gene Description
DnaJ (Hsp40) homolog, subfamily B, member 4
Omim ID
611327Gene Ontology
HyperlinkGene Summary
DNAJB4 belongs to the evolutionarily conserved DNAJ/HSP40 protein family. For background information on the DNAJ family, see MIM 608375.[supplied by OMIM
Other Designations
DnaJ-like heat shock protein 40|OTTHUMP00000038541
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