C6orf32 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-C6orf32 full length
Host
Human
Theoretical MW (kDa)
66
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-C6orf32 antibody (H00009750-M02) by Western Blots.
SDS-PAGE Gel
C6orf32 transfected lysate
Western Blot
Lane 1: C6orf32 transfected lysate ( 66 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — FAM65B
Entrez GeneID
9750GeneBank Accession#
BC001232Protein Accession#
AAH01232Gene Name
FAM65B
Gene Alias
C6orf32, DIFF40, DIFF48, KIAA0386, PL48
Gene Description
family with sequence similarity 65, member B
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene stimulates the formation of a non-mitotic multinucleate syncytium from proliferative cytotrophoblasts during trophoblast differentiation. Two alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
hypothetical protein LOC9750
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