BCL7B HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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More Files
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-BCL7B full length
Host
Human
Theoretical MW (kDa)
22
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-BCL7B antibody (H00009275-M01) by Western Blots.
SDS-PAGE Gel
BCL7B transfected lysate
Western Blot
Lane 1: BCL7B transfected lysate ( 22 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — BCL7B
Entrez GeneID
9275GeneBank Accession#
NM_001707Protein Accession#
NP_001698Gene Name
BCL7B
Gene Alias
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Gene Description
B-cell CLL/lymphoma 7B
Omim ID
605846Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene contains a region that is highly similar to the N-terminal segment of BCL7A protein. The BCL7A protein is encoded by the gene known to be directly involved in a three-way gene translocation in a Burkitt lymphoma cell line. This gene is located at a chromosomal region commonly deleted in Williams syndrome. The function of this gene has not yet been determined. [provided by RefSeq
Other Designations
OTTHUMP00000025030
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Interactome
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