ANXA3 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

Catalog # L017T6

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Size:100 ug
Price: USD $ 247.00
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Images
SDS-PAGE Gel
QC Test

SDS-PAGE Gel

ANXA3 transfected lysate

Western Blot
QC Test

Western Blot

Lane 1: ANXA3 transfected lysate ( 36 KDa).
Lane 2: Non-transfected lysate.

  • Specification

    Transfected Cell Line

    HEK293

    Plasmid

    pCMV-ANXA3 full length

    Host

    Human

    Theoretical MW (kDa)

    36

    Lysis Buffer

    Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

    Concentration

    2 mg/ml

    Quality Control Testing

    Transient overexpression cell lysate was tested with Anti-ANXA3 antibody (H00000306-M12) by Western Blots.

    SDS-PAGE Gel

    ANXA3 transfected lysate

    Western Blot

    Lane 1: ANXA3 transfected lysate ( 36 KDa).
    Lane 2: Non-transfected lysate.

    Recommend Usage

    Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.

    Storage Buffer

    In modified RIPA Lysis Buffer.

    Storage Instruction

    Store at -80°C. Aliquot to avoid repeated freezing and thawing.

  • Applications

    Western Blot

    Immunoprecipitation

  • Gene Info — ANXA3

    Entrez GeneID

    306

    GeneBank Accession#

    BC000871

    Protein Accession#

    AAH00871

    Gene Name

    ANXA3

    Gene Alias

    ANX3

    Gene Description

    annexin A3

    Omim ID

    106490

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phopholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation. [provided by RefSeq

    Other Designations

    Annexin III (lipocortin III)|annexin III (lipocortin III, 1,2-cyclic-inositol-phosphate phosphodiesterase, placental anticoagulant protein III, calcimedin 35-alpha)|calcimedin 35-alpha

  • Interactome
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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