miRNA Tissue / Cultured Cells Isolation Kit
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Total RNA from mouse tissue was isolated using a reagent system. 10 μg of each RNA was subjected to miRNA isolation using the miRNA Tissue / Cultured Cells Isolation Kit. The purified miRNA was resolved in 50 μL of Release Buffer and a 1/10 volume aliquot (5 μL) was analyzed by electrophoresis on a 2% agarose gel.
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Specification
Product Description
miRNA Tissue / Cultured Cells Isolation Kit is designed for purifying and enriching micro RNAs (miRNAs) and other small cellular RNAs from a wide variety of tissues and cells.
Supplied Product
Kit content:
1. Lysis Buffer
2. Mi Buffer
3. Wash Buffer
4. Release Buffer
5. RNA Columns
6. 2 mL Collection Tubes
7. MicropestlesPack Size
50 reactions
Suitable Sample
Tissue, cultured cells
Sample Size
100 mg of tissue, 1 x 106 cultured cells
Regulation Status
For research use only (RUO)
Quality Control Testing
Electrophoresis
Total RNA from mouse tissue was isolated using a reagent system. 10 μg of each RNA was subjected to miRNA isolation using the miRNA Tissue / Cultured Cells Isolation Kit. The purified miRNA was resolved in 50 μL of Release Buffer and a 1/10 volume aliquot (5 μL) was analyzed by electrophoresis on a 2% agarose gel.
Storage Instruction
Store at room temperature (15-25°C).
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Applications
Purification
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