Rho-kinase (Human) Assay Kit
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Specification
Product Description
Rho-kinase (Human) Assay Kit is a single-site, non-quantitative immunoassay for Rho-kinase activity. Plates are pre-coated with a substrate corresponding to recombinant the C terminus of MBS (Myosin-Binding Subunit of myosin phosphatase), which contains a threonine residue that may be phosphorylated by DMPK family members, including Rho-kinase (ROCK1 and ROCK2), MRCK (Myotonic Dystrophy kinase-related Cdc42-binding Kinase) and DMPK (Myotonic Dystrophy Protein Kinase). The detector antibody is AF20, an antibody that specifically detects only the phosphorylated form of threonine-696 on MBS.
Assay Type
Quantitative
Reactivity
Human
Regulation Status
For research use only (RUO)
Quality Control Testing
Dose dependent curve
Dose dependent curve of rho kinase-II catalytic domain enzyme reaction.
Storage Instruction
Store the kit at 4°C.
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Applications
Functional Study
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Publication Reference
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Thrombin-induced endothelial microparticle generation: identification of a novel pathway involving ROCK-II activation by caspase-2.
Sapet C, Simoncini S, Loriod B, Puthier D, Sampol J, Nguyen C, Dignat-George F, Anfosso F.
Blood 2006 Sep; 108(6):1868.
Application:Func, Human, HMEC-1 cells.
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Inhibition of Rho-kinase affects astrocytoma morphology, motility, and invasion through activation of Rac1.
Salhia B, Rutten F, Nakada M, Beaudry C, Berens M, Kwan A, Rutka JT.
Cancer Research 2005 Oct; 65(19):8792.
Application:Func, Human, U251, U87 cells.
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Simvastatin attenuates expression of cytokine-inducible nitric-oxide synthase in embryonic cardiac myoblasts.
Madonna R, Di Napoli P, Massaro M, Grilli A, Felaco M, De Caterina A, Tang D, De Caterina R, Geng YJ.
The Journal of Biological Chemistry 2005 Apr; 280(14):13503.
Application:Func, Rat, H9c2 cardiac myoblasts.
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Impact of engagement of FcepsilonRI and CC chemokine receptor 1 on mast cell activation and motility.
Toda M, Dawson M, Nakamura T, Munro PM, Richardson RM, Bailly M, Ono SJ.
The Journal of Biological Chemistry 2004 Nov; 279(46):48443.
Application:Func, Rat, RBL-CCR1 cells.
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The Sprouty-related protein, Spred, inhibits cell motility, metastasis, and Rho-mediated actin reorganization.
Miyoshi K, Wakioka T, Nishinakamura H, Kamio M, Yang L, Inoue M, Hasegawa M, Yonemitsu Y, Komiya S, Yoshimura A.
Oncogene 2004 Jul; 23(33):5567.
Application:Func, Human, HEK 293 cells.
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Thrombin-induced endothelial microparticle generation: identification of a novel pathway involving ROCK-II activation by caspase-2.
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