SUMO4 monoclonal antibody (M02), clone 1D3
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of SUMO4 expression in transfected 293T cell line by SUMO4 monoclonal antibody (M02), clone 1D3.
Lane 1: SUMO4 transfected lysate (Predicted MW: 10.7 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SUMO4 is 0.1 ng/ml as a capture antibody.
Western Blot detection against Immunogen (37.1 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant SUMO4.
Immunogen
SUMO4 (NP_001002255.1, 1 a.a. ~ 95 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MANEKPTEEVKTENNNHINLKVAGQDGSVVQFKIKRQTPLSKLMKAYCEPRGLSVKQIRFRFGGQPISGTDKPAQLEMEDEDTIDVFQQPTGGVY
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.1 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of SUMO4 expression in transfected 293T cell line by SUMO4 monoclonal antibody (M02), clone 1D3.
Lane 1: SUMO4 transfected lysate (Predicted MW: 10.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SUMO4 is 0.1 ng/ml as a capture antibody.ELISA
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Gene Info — SUMO4
Entrez GeneID
387082GeneBank Accession#
NM_001002255.1Protein Accession#
NP_001002255.1Gene Name
SUMO4
Gene Alias
IDDM5, SMT3H4, SUMO-4, dJ281H8.4
Gene Description
SMT3 suppressor of mif two 3 homolog 4 (S. cerevisiae)
Gene Ontology
HyperlinkGene Summary
This gene is a member of the SUMO gene family. This family of genes encode small ubiquitin-related modifiers that are attached to proteins and control the target proteins' subcellular localization, stability, or activity. The protein described in this record is located in the cytoplasm and specifically modifies IKBA, leading to negative regulation of NF-kappa-B-dependent transcription of the IL12B gene. A specific polymorphism in this SUMO gene, which leads to the M55V substitution, has been associated with type I diabetes. The RefSeq contains this polymorphism. [provided by RefSeq
Other Designations
OTTHUMP00000017390|SMT3 suppressor of mif two 3 homolog 2|SMT3 suppressor of mif two 3 homolog 4|small ubiquitin-like modifier 4 protein|small ubiquitin-like protein 4
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Disease
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