NMNAT3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
NMNAT3 transfected lysate.
Western Blot
Lane 1: NMNAT3 transfected lysate ( 23.76 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NMNAT3 full-length
Host
Human
Theoretical MW (kDa)
23.76
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NMNAT3 antibody (H00349565-B01) by Western Blots.
SDS-PAGE Gel
NMNAT3 transfected lysate.
Western Blot
Lane 1: NMNAT3 transfected lysate ( 23.76 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NMNAT3
Entrez GeneID
349565GeneBank Accession#
NM_178177.2Protein Accession#
NP_835471.1Gene Name
NMNAT3
Gene Alias
PNAT-3, PNAT3
Gene Description
nicotinamide nucleotide adenylyltransferase 3
Omim ID
608702Gene Ontology
HyperlinkGene Summary
The coenzyme NAD and its derivatives are involved in hundreds of metabolic redox reactions and are utilized in protein ADP-ribosylation, histone deacetylation, and in some Ca(2+) signaling pathways. NMNAT (EC 2.7.7.1) is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD (Zhang et al., 2003 [PubMed 12574164]).[supplied by OMIM
Other Designations
pyridine nucleotide adenylyltransferase 3
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