FBXW12 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
FBXW12 transfected lysate.
Western Blot
Lane 1: FBXW12 transfected lysate ( 49.06 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-FBXW12 full-length
Host
Human
Theoretical MW (kDa)
49.06
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-FBXW12 antibody (H00285231-B01) by Western Blots.
SDS-PAGE Gel
FBXW12 transfected lysate.
Western Blot
Lane 1: FBXW12 transfected lysate ( 49.06 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — FBXW12
Entrez GeneID
285231GeneBank Accession#
BC101305.1Protein Accession#
AAI01306.1Gene Name
FBXW12
Gene Alias
FBXO12, FBXO35, Fbw12, MGC120385, MGC120386, MGC120387
Gene Description
F-box and WD repeat domain containing 12
Omim ID
609075Gene Ontology
HyperlinkGene Summary
Members of the F-box protein family, such as FBXW12, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603034), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM
Other Designations
F-box and WD-40 domain protein 12|F-box only protein 35|F-box- and WD40-repeat-containing protein|OTTHUMP00000164809
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