SENP5 monoclonal antibody (M01), clone 3C2
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of SENP5 expression in transfected 293T cell line by SENP5 monoclonal antibody (M01), clone 3C2.
Lane 1: SENP5 transfected lysate(86.7 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of monoclonal antibody to SENP5 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (37.62 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant SENP5.
Immunogen
SENP5 (NP_689912, 2 a.a. ~ 109 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
KKQRKILWRKGIHLAFSEKWNTGFGGFKKFYFHQHLCILKAKLGRPVTWNRQLRHFQGRKKALQIQKTWIKDEHLCAKTKFNVATQNVSTLSSKVKRKDAKHFISSSK
Host
Mouse
Reactivity
Human
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.62 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of SENP5 expression in transfected 293T cell line by SENP5 monoclonal antibody (M01), clone 3C2.
Lane 1: SENP5 transfected lysate(86.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to SENP5 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — SENP5
Entrez GeneID
205564GeneBank Accession#
NM_152699Protein Accession#
NP_689912Gene Name
SENP5
Gene Alias
DKFZp564O1016, FLJ42398, MGC27076
Gene Description
SUMO1/sentrin specific peptidase 5
Gene Ontology
HyperlinkGene Summary
The reversible posttranslational modification of proteins by the addition of small ubiquitin-like SUMO proteins (see SUMO1; MIM 601912) is required for numerous biologic processes. SUMO-specific proteases, such as SENP5, are responsible for the initial processing of SUMO precursors to generate a C-terminal diglycine motif required for the conjugation reaction. They also have isopeptidase activity for the removal of SUMO from high molecular mass SUMO conjugates (Di Bacco et al., 2006 [PubMed 16738315]).[supplied by OMIM
Other Designations
SUMO1/sentrin specific protease 5
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Interactome
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