C9orf72 (Human) Recombinant Protein (P01)
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More Files
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Specifications
Product Description
Human C9orf72 full-length ORF (NP_060795.1, 1 a.a. - 481 a.a.) recombinant protein with GST tag at N-terminal.
Sequence
MSTLCPPPSPAVAKTEIALSGKSPLLAATFAYWDNILGPRVRHIWAPKTEQVLLSDGEITFLANHTLNGEILRNAESGAIDVKFFVLSEKGVIIVSLIFDGNWNGDRSTYGLSIILPQTELSFYLPLHRVCVDRLTHIIRKGRIWMHKERQENVQKIILEGTERMEDQGQSIIPMLTGEVIPVMELLSSMKSHSVPEEIDIADTVLNDDDIGDSCHEGFLLNAISSHLQTCGCSVVVGSSAEKVNKIVRTLCLFLTPAERKCSRLCEAESSFKYESGLFVQGLLKDSTGSFVLPFRQVMYAPYPTTHIDVDVNTVKQMPPCHEHIYNQRRYMRSELTAFWRATSEEDMAQDTIIYTDESFTPDLNIFQDVLHRDTLVKAFLDQVFQLKPGLSLRSTFLAQFLLVLHRKALTLIKYIEDDTQKGKKPFKSLRNLKIDLDLTAEGDLNIIMALAEKIKPGLHSFIFGRPFYTSVQERDVLMTF
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
80.7
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — C9orf72
Entrez GeneID
203228GeneBank Accession#
NM_018325.1Protein Accession#
NP_060795.1Gene Name
C9orf72
Gene Alias
MGC23980, RP11-27J8.2
Gene Description
chromosome 9 open reading frame 72
Gene Ontology
HyperlinkOther Designations
OTTHUMP00000021170|OTTHUMP00000021171|OTTHUMP00000021172|OTTHUMP00000045179|hypothetical protein LOC203228
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Interactomes
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Diseases
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Publication Reference
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New Antibody-Free Mass Spectrometry-Based Quantification Reveals That C9ORF72 Long Protein Isoform Is Reduced in the Frontal Cortex of Hexanucleotide-Repeat Expansion Carriers.
Viodé A, Fournier C, Camuzat A, Fenaille F; NeuroCEB Brain Bank, Latouche M, Elahi F, Ber IL, Junot C, Lamari F, Anquetil V, Becher F.
Frontiers in Neuroscience 2018 Aug; 12:589.
Application:Func, Human, Human brains.
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A complex of C9ORF72 and p62 uses arginine methylation to eliminate stress granules by autophagy.
Chitiprolu M, Jagow C, Tremblay V, Bondy-Chorney E, Paris G, Savard A, Palidwor G, Barry FA, Zinman L, Keith J, Rogaeva E, Robertson J, Lavallée-Adam M, Woulfe J, Couture JF, Côté J, Gibbings D.
Nature Communications 2018 Jul; 9(1):2794.
Application:PI, WB-Re, Recombinant protein.
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New Antibody-Free Mass Spectrometry-Based Quantification Reveals That C9ORF72 Long Protein Isoform Is Reduced in the Frontal Cortex of Hexanucleotide-Repeat Expansion Carriers.
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