DNAJB8 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DNAJB8 full-length
Host
Human
Theoretical MW (kDa)
25.7
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DNAJB8 antibody (H00165721-B01P) by Western Blots.
SDS-PAGE Gel
DNAJB8 transfected lysate.
Western Blot
Lane 1: DNAJB8 transfected lysate ( 25.70 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DNAJB8
Entrez GeneID
165721GeneBank Accession#
NM_153330Protein Accession#
NP_699161.1Gene Name
DNAJB8
Gene Alias
DJ6, MGC33884
Gene Description
DnaJ (Hsp40) homolog, subfamily B, member 8
Omim ID
611337Gene Ontology
HyperlinkGene Summary
DNAJB8 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000 [PubMed 11147971]).[supplied by OMIM
Other Designations
DnaJ homolog, subfamily B, member 8
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