H1FOO 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
H1FOO transfected lysate.
Western Blot
Lane 1: H1FOO transfected lysate ( 21 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-H1FOO full-length
Host
Human
Theoretical MW (kDa)
21
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-H1FOO antibody (H00132243-B01) by Western Blots.
SDS-PAGE Gel
H1FOO transfected lysate.
Western Blot
Lane 1: H1FOO transfected lysate ( 21 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — H1FOO
Entrez GeneID
132243GeneBank Accession#
BC047943Protein Accession#
AAH47943Gene Name
H1FOO
Gene Alias
MGC50807, osH1
Gene Description
H1 histone family, member O, oocyte-specific
Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein encoded is a member of the histone H1 family. This gene contains introns, unlike most histone genes. The protein encoded is a member of the histone H1 family. The related mouse gene is expressed only in oocytes. [provided by RefSeq
Other Designations
oocyte-specific histone H1
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