MED8 rabbit monoclonal antibody
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Specification
Product Description
Rabbit monoclonal antibody raised against a human MED8 peptide using ARM Technology.
Immunogen
A synthetic peptide of human MED8 is used for rabbit immunization.
Customer or Abnova will decide on the preferred peptide sequence.Host
Rabbit
Library Construction
Non-fusion antibody library from rabbit spleen (ARM Technology).
Expression
Overexpression vector and transfection into 293H cell line.
Reactivity
Human
Purification
Protein A
Isotype
IgG
Quality Control Testing
Antibody reactive against human MED8 peptide by ELISA and mammalian transfected lysate by Western Blot.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Deliverable
Up to three rabbit IgG clones of 100 ug each will be delivered to customer.
Note
1. Customer may provide cell or tissue lysate for antibody screening.
2. Rabbit monoclonal antibody generated by ARM technology is amenable to antibody engineering including F(ab)2, IgG, scFv and different Fc and non-Fc conjugates per customer request. -
Applications
Western Blot (Transfected lysate)
ELISA
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Gene Info — MED8
Entrez GeneID
112950GeneBank Accession#
MED8Gene Name
MED8
Gene Alias
ARC32, MGC17544, MGC19641
Gene Description
mediator complex subunit 8
Omim ID
607956Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is one of more than 20 subunits of the mediator complex, first identified in S. cerevisiae, that is required for activation of transcription. The product of this gene also interacts with elongins B and C, and CUL2 and RBX1, to reconstitute a ubiquitin ligase. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000008573|OTTHUMP00000008581|activator-recruited cofactor 32 kDa component|mediator of RNA polymerase II transcription subunit MED8|mediator of RNA polymerase II transcription, subunit 8 homolog
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Interactome
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