ARHGAP12 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ARHGAP12 transfected lysate.
Western Blot
Lane 1: ARHGAP12 transfected lysate ( 95.8 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ARHGAP12 full-length
Host
Human
Theoretical MW (kDa)
95.8
Interspecies Antigen Sequence
Mouse (91); Rat (92)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ARHGAP12 antibody (H00094134-B01) by Western Blots.
SDS-PAGE Gel
ARHGAP12 transfected lysate.
Western Blot
Lane 1: ARHGAP12 transfected lysate ( 95.8 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ARHGAP12
Entrez GeneID
94134GeneBank Accession#
BC094719.1Protein Accession#
AAH94719.1Gene Name
ARHGAP12
Gene Alias
DKFZp779N2050, FLJ10971, FLJ20737, FLJ21785, FLJ45709
Gene Description
Rho GTPase activating protein 12
Omim ID
610577Gene Ontology
HyperlinkGene Summary
RHO GTPases (see ARHA, MIM 165390) that participate in a wide variety of cellular processes are active in their GTP-bound form and inactive in their GDP-bound form. The hydrolysis of GTP and the inactivation of these GTPases are regulated by GTPase-activating proteins (GAPs), such as ARHGAP12 (Zhang et al., 2002 [PubMed 11854031]).[supplied by OMIM
Other Designations
OTTHUMP00000019410
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