ALG2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ALG2 transfected lysate.
Western Blot
Lane 1: ALG2 transfected lysate ( 45.87 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ALG2 full-length
Host
Human
Theoretical MW (kDa)
45.87
Interspecies Antigen Sequence
Mouse (81); Rat (81)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ALG2 antibody (H00085365-B01) by Western Blots.
SDS-PAGE Gel
ALG2 transfected lysate.
Western Blot
Lane 1: ALG2 transfected lysate ( 45.87 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ALG2
Entrez GeneID
85365GeneBank Accession#
NM_033087.3Protein Accession#
NP_149078.1Gene Name
ALG2
Gene Alias
CDGIi, FLJ14511, hALPG2
Gene Description
asparagine-linked glycosylation 2, alpha-1,3-mannosyltransferase homolog (S. cerevisiae)
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the glycosyltransferase 1 family. The encoded protein acts as an alpha 1,3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii). Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
GDP-Man:Man(1)GlcNAc(2)-PP-dolichol mannosyltransferase|OTTHUMP00000021785|OTTHUMP00000123474|alpha-1,3-mannosyltransferase ALG2|asparagine-linked glycosylation 2 homolog (S. cerevisiae, alpha-1,3-mannosyltransferase)|asparagine-linked glycosylation 2 hom
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Interactome
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Pathway
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