ITCH 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ITCH transfected lysate.
Western Blot
Lane 1: ITCH transfected lysate ( 94.93 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ITCH full-length
Host
Human
Theoretical MW (kDa)
94.93
Interspecies Antigen Sequence
Mouse (96); Rat (95)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ITCH antibody (H00083737-B01) by Western Blots.
SDS-PAGE Gel
ITCH transfected lysate.
Western Blot
Lane 1: ITCH transfected lysate ( 94.93 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ITCH
Entrez GeneID
83737GeneBank Accession#
NM_031483.3Protein Accession#
NP_113671.3Gene Name
ITCH
Gene Alias
AIF4, AIP4, NAPP1, dJ468O1.1
Gene Description
itchy E3 ubiquitin protein ligase homolog (mouse)
Omim ID
606409Gene Ontology
HyperlinkGene Summary
Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells. [provided by RefSeq
Other Designations
NFE2-associated polypeptide 1|atrophin-1 interacting protein 4|dJ468O1.1 (atrophin 1 interacting protein 4 (AIP4))|itchy homolog E3 ubiquitin protein ligase|ubiquitin protein ligase ITCH
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Interactome
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Pathway
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