RASSF5 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
RASSF5 transfected lysate
Western Blot
Lane 1: RASSF5 transfected lysate ( 30.4 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RASSF5 full-length
Host
Human
Theoretical MW (kDa)
30.4
Interspecies Antigen Sequence
Mouse (94); Rat (93)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RASSF5 antibody (H00083593-B01) by Western Blots.
SDS-PAGE Gel
RASSF5 transfected lysate
Western Blot
Lane 1: RASSF5 transfected lysate ( 30.4 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RASSF5
Entrez GeneID
83593GeneBank Accession#
NM_182665Protein Accession#
NP_872606Gene Name
RASSF5
Gene Alias
MGC10823, MGC17344, Maxp1, NORE1, NORE1A, NORE1B, RAPL, RASSF3
Gene Description
Ras association (RalGDS/AF-6) domain family member 5
Omim ID
607020Gene Ontology
HyperlinkGene Summary
This gene is a member of the Ras association domain family. It functions as a tumor suppressor, and is inactivated in a variety of cancers. The encoded protein localizes to centrosomes and microtubules, and associates with the GTP-activated forms of Ras, Rap1, and several other Ras-like small GTPases. The protein regulates lymphocyte adhesion and suppresses cell growth in response to activated Rap1 or Ras. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000034534|OTTHUMP00000034535|Rap1-binding protein|Ras association (RalGDS/AF-6) domain family 5|Ras effector-like protein|novel Ras effector 1|regulator for cell adhesion and polarization enriched in lymphoid tissue|tumor suppressor RASSF3
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Interactome
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Pathway
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Disease
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