GSDMD monoclonal antibody (M01), clone 3F12-1B2
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant GSDMD.
Immunogen
GSDMD (AAH08904, 1 a.a. ~ 484 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MGSAFERVVRRVVQELDHGGEFIPVTSLQSSTGFQPYCLVVRKPSSSWFWKPRYKCVNLSIKDILEPDAAEPDVQRGRSFHFYDAMDGQIQGSVELAAPGQAKIAGGAAVSDSSSTSMNVYSLSVDPNTWQTLLHERHLRQPEHKVLQQLRSRGDNVYVVTEVLQTQKEVEVTRTHKREGSGRFSLPGATCLQGEGQGHLSQKKTVTIPSGSTLAFRVAQLVIDSDLDVLLFPDKKQRTFQPPATGHKRSTSEGAWPQLPSGLSMMRCLHNFLTDGVPAEGAFTEDFQGLRAEVETISKELELLDRELCQLLLEGLEGVLRDQLALRALEEALEQGQSLGPVEPLDGPAGAVLECLVLSSGMLVPELAIPVVYLLGALTMLSETQHKLLAEALESQTLLGPLELVGSLLEQSAPWQERSTMSLPPGLLGNSWGEGAPAWVLLDECGLELGEDTPHVCWEPQAQGRMCALYASLALLSGLSQEPH
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (57); Rat (54)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (78.98 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
GSDMD monoclonal antibody (M01), clone 3F12-1B2 Western Blot analysis of GSDMD expression in Jurkat ( Cat # L017V1 ).Western Blot (Transfected lysate)
Western Blot analysis of GSDMD expression in transfected 293T cell line by GSDMD monoclonal antibody (M01), clone 3F12-1B2.
Lane 1: GSDMD transfected lysate (Predicted MW: 52.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to GSDMD on formalin-fixed paraffin-embedded human tonsil tissue. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GSDMD is approximately 0.3ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to GSDMD on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — GSDMD
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Interactome
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Publication Reference
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Gasdermin D Represses Inflammation-Induced Colon Cancer Development by Regulating Apoptosis.
Shigekazu Tanaka, Hajime Orita, Taro Kataoka, Masahiro Miyazaki, Harumi Saeki, Ryo Wada, Malcolm V Brock, Tetsu Fukunaga, Takanori Amano, Toshihiko Shiroishi.
Carcinogenesis 2023 Feb; bgad005.
Application:IHC-P, Human, Mouse, Human colon, Mouse colon.
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Regulation of Mesenchymal Cell Fate by Transfer of Active Gasdermin-D via Monocyte-Derived Extracellular Vesicles.
Anasuya Sarkar, Srabani Das, Hannah Bone, Ivana DeVengencie, Jayendra Prasad, Daniela Farkas, James D Londino, Richard S Nho, Mauricio Rojas, Jeffrey C Horowitz.
Journal of immunology 2023 Jan; ji2200511.
Application:WB-Ce, WB-Tr, Human, Human lung fibroblasts, THP-1 cells.
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Brain immune cells undergo cGAS-STING-dependent apoptosis during herpes simplex virus type 1 infection.
Line S Reinert, Ahmad S Rashidi, Diana N Tran, Georgios Katzilieris-Petras, Astrid K Hvidt, Mette Gohr, Stefanie Fruhwürth, Chiranjeevi Bodda, Martin K Thomsen, Mikkel H Vendelbo, Ahmad Raza Khan, Brian Hansen, Petra Bergström, Lotta Agholme, Trine H Mogensen, Maria H Christensen, Jens R Nyengaard, Ganes C Sen, Henrik Zetterberg, Georges Mgm Verjans, Soren R Paluden.
The Journal of Clinical Investigation 2020 Sep; 136824.
Application:IHC, Human, Human brain.
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Gasdermin D Represses Inflammation-Induced Colon Cancer Development by Regulating Apoptosis.
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