THTPA monoclonal antibody (M01), clone 3F6
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More Files
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Western Blot (Cell lysate)
THTPA monoclonal antibody (M01), clone 3F6 Western Blot analysis of THTPA expression in A-431 ( Cat # L015V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of THTPA expression in transfected 293T cell line by THTPA monoclonal antibody (M01), clone 3F6.
Lane 1: THTPA transfected lysate(25.6 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to THTPA on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged THTPA is approximately 0.1ng/ml as a capture antibody.
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant THTPA.
Immunogen
THTPA (AAH02984, 1 a.a. ~ 230 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAQGLIEVERKFLPGPGTEERLQELGGTLEYRVTFRDTYYDTPELSLMQADHWLRRREDSGWELKCPGAAGVLGPHTEYKELTAEPTIVAQLCKVLRADGLGAGDVAAVLGPLGLQEVASFVTKRSAWKLVLLGADEEEPQLRVDLDTADFGYAVGEVEALVHEEAEVPTALEKIHRLSSMLGVPAQETAPAKLIVYLQRFRPQDYQRLLEVNSSRERPQETEDPDHCLG
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (74); Rat (73)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
THTPA monoclonal antibody (M01), clone 3F6 Western Blot analysis of THTPA expression in A-431 ( Cat # L015V1 ).Western Blot (Transfected lysate)
Western Blot analysis of THTPA expression in transfected 293T cell line by THTPA monoclonal antibody (M01), clone 3F6.
Lane 1: THTPA transfected lysate(25.6 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to THTPA on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged THTPA is approximately 0.1ng/ml as a capture antibody.ELISA
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Gene Info — THTPA
Entrez GeneID
79178GeneBank Accession#
BC002984Protein Accession#
AAH02984Gene Name
THTPA
Gene Alias
MGC2652, THTP, THTPASE
Gene Description
thiamine triphosphatase
Omim ID
611612Gene Ontology
HyperlinkGene Summary
Thiamine triphosphatase (THTPA) catalyzes the hydrolysis of thiamine triphosphate (THTP) to thiamine diphosphate (THDP), which is the major cellular form of thiamine (vitamin B1) (Lakaye et al., 2002 [PubMed 11827967]).[supplied by OMIM
Other Designations
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Interactome
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Pathway
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Publication Reference
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Thiamine status in humans and content of phosphorylated thiamine derivatives in biopsies and cultured cells.
Gangolf M, Czerniecki J, Radermecker M, Detry O, Nisolle M, Jouan C, Martin D, Chantraine F, Lakaye B, Wins P, Grisar T, Bettendorff L.
PLoS One 2010 Oct; 5(10):e13616.
Application：WB-Ti, WB-Ce, Human, Ectocervix; Endometrium, Human recombinant ThTPase, Human LN-18 glioblastoma cells, Human SK-N-BE neuroblastoma cells, Trophoblasts, Uterus.
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The Iron-Regulated Metastasis Suppressor, Ndrg-1: Identification of Novel Molecular Targets.
Kovacevic Z, Fu D, Richardson DR.
Biochimica et Biophysica Acta 2008 Jun; 1783(10):1981.
Application：WB-Tr, Human, DLD-1, NCI-H1299, MIAPaCa-2 cells.
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Thiamine status in humans and content of phosphorylated thiamine derivatives in biopsies and cultured cells.
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