WNK4 (Human) Recombinant Protein (Q01)
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Specification
Product Description
Human WNK4 partial ORF ( NP_115763.2, 1144 a.a. - 1243 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
KHLSEVETLQTLQKKEIEDLYSRLGKQPPPGIVAPAAMLSSRQRRLSKGSFPTSRRNSLQRSEPPGPGIMRRNSLSGSSTGSQEQRASKGVTFAGDVGRM
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
36.74
Interspecies Antigen Sequence
Mouse (95); Rat (95)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — WNK4
Entrez GeneID
65266GeneBank Accession#
NM_032387Protein Accession#
NP_115763.2Gene Name
WNK4
Gene Alias
PHA2B, PRKWNK4
Gene Description
WNK lysine deficient protein kinase 4
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the WNK family of serine-threonine protein kinases. The kinase is part of the tight junction complex in kidney cells, and regulates the balance between NaCl reabsorption and K(+) secretion. The kinase regulates the activities of several types of ion channels, cotransporters, and exchangers involved in electrolyte flux in epithelial cells. Mutations in this gene result in pseudohypoaldosteronism type IIB
Other Designations
protein kinase, lysine deficient 4
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Disease
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