UPF3A 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
UPF3A transfected lysate.
Western Blot
Lane 1: UPF3A transfected lysate ( 48.84 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-UPF3A full-length
Host
Human
Theoretical MW (kDa)
48.84
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-UPF3A antibody (H00065110-B01) by Western Blots.
SDS-PAGE Gel
UPF3A transfected lysate.
Western Blot
Lane 1: UPF3A transfected lysate ( 48.84 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — UPF3A
Entrez GeneID
65110GeneBank Accession#
NM_080687.1Protein Accession#
NP_542418.1Gene Name
UPF3A
Gene Alias
HUPF3A, RENT3A, UPF3
Gene Description
UPF3 regulator of nonsense transcripts homolog A (yeast)
Omim ID
605530Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome 13. Two splice variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000018789|UPF3 regulator of nonsense transcripts homolog A
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