CERK 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CERK transfected lysate.
Western Blot
Lane 1: CERK transfected lysate ( 21.7 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CERK full-length
Host
Human
Theoretical MW (kDa)
22.22
Interspecies Antigen Sequence
Mouse (100); Rat (100)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CERK antibody (H00064781-B01) by Western Blots.
SDS-PAGE Gel
CERK transfected lysate.
Western Blot
Lane 1: CERK transfected lysate ( 21.7 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CERK
Entrez GeneID
64781GeneBank Accession#
NM_182661Protein Accession#
NP_872602Gene Name
CERK
Gene Alias
DKFZp434E0211, FLJ21430, FLJ23239, KIAA1646, LK4, MGC131878, dA59H18.2, dA59H18.3, hCERK
Gene Description
ceramide kinase
Omim ID
610307Gene Ontology
HyperlinkGene Summary
CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation (Kim et al., 2006 [PubMed 16488390]).[supplied by OMIM
Other Designations
OTTHUMP00000028554|lipid kinase LK4
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Interactome
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