ACSS2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ACSS2 transfected lysate.
Western Blot
Lane 1: ACSS2 transfected lysate ( 66.77 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ACSS2 full-length
Host
Human
Theoretical MW (kDa)
66.77
Interspecies Antigen Sequence
Mouse (93); Rat (93)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ACSS2 antibody (H00055902-B01) by Western Blots.
SDS-PAGE Gel
ACSS2 transfected lysate.
Western Blot
Lane 1: ACSS2 transfected lysate ( 66.77 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ACSS2
Entrez GeneID
55902GeneBank Accession#
NM_139274.1Protein Accession#
AAH98422.1Gene Name
ACSS2
Gene Alias
ACAS2, ACS, ACSA, AceCS, DKFZp762G026, dJ1161H23.1
Gene Description
acyl-CoA synthetase short-chain family member 2
Omim ID
605832Gene Ontology
HyperlinkGene Summary
This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
OTTHUMP00000030712|OTTHUMP00000030713|OTTHUMP00000030714|OTTHUMP00000030715|OTTHUMP00000030716|acetate thiokinase|acetate-CoA ligase|acetyl-CoA synthetase|acetyl-Coenzyme A synthetase 2 (ADP forming)|acyl-activating enzyme|cytoplasmic acetyl-coenzyme A sy
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Interactome
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Pathway
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Disease
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