FLJ20643 monoclonal antibody (M01), clone 4F5-1E10
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of PIH1D1 expression in transfected 293T cell line by FLJ20643 monoclonal antibody (M01), clone 4F5-1E10.
Lane 1: PIH1D1 transfected lysate (Predicted MW: 32.4 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of PIH1D1 transfected lysate using anti-PIH1D1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PIH1D1 MaxPab rabbit polyclonal antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to PIH1D1 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (57.64 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant FLJ20643.
Immunogen
FLJ20643 (AAH01108, 1 a.a. ~ 290 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MANPKLLGLELSEAEAIGADSARFEELLLQASKELQQAQTTRPESTQIQPQPGFCIKTNSSEGKVFINICHSPSIPPPADVTEEELLQMLEEDQAGFRIPMSLGEPHAELDAKGQGCTAYDVAVNSDFYRRMQNSDFLRELVITIAREGLEDKYNLQLNPEWRMMKNRPFMGSISQQNIRSEQRPRIQELGDLYTPAPGRAESGPEKPHLNLWLEAPDLLLAEIDLPKLDGALGLSLEIGENRLVMGGPQQLYHLDAYIPLQINSHESKAAFHRKRKQLMVAMPLLLVPS
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (80); Rat (81)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (57.64 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PIH1D1 expression in transfected 293T cell line by FLJ20643 monoclonal antibody (M01), clone 4F5-1E10.
Lane 1: PIH1D1 transfected lysate (Predicted MW: 32.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of PIH1D1 transfected lysate using anti-PIH1D1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PIH1D1 MaxPab rabbit polyclonal antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to PIH1D1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — PIH1D1
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Interactome
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Publication Reference
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DPCD is a regulator of R2TP in ciliogenesis initiation through Akt signaling.
Yu-Qian Mao, Thiago V Seraphim, Yimei Wan, Ruikai Wu, Etienne Coyaud, Muhammad Bin Munim, Antonio Mollica, Estelle Laurent, Mohan Babu, Vito Mennella, Brian Raught, Walid A Houry.
Cell Reports 2024 Feb; 43(2):113713.
Application：IF, Human, RPE-1 cells.
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DPCD is a regulator of R2TP in ciliogenesis initiation through Akt signaling.
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