SIAE 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
SIAE transfected lysate.
Western Blot
Lane 1: SIAE transfected lysate ( 57.64 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SIAE full-length
Host
Human
Theoretical MW (kDa)
57.64
Interspecies Antigen Sequence
Mouse (76); Rat (75)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SIAE antibody (H00054414-B01) by Western Blots.
SDS-PAGE Gel
SIAE transfected lysate.
Western Blot
Lane 1: SIAE transfected lysate ( 57.64 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SIAE
Entrez GeneID
54414GeneBank Accession#
NM_170601.3Protein Accession#
NP_733746.1Gene Name
SIAE
Gene Alias
CSE-C, LSE, MGC87009, YSG2
Gene Description
sialic acid acetylesterase
Omim ID
610079Gene Ontology
HyperlinkGene Summary
Sialic acids are acidic 9-carbon sugars typically found at the nonreducing end of sugar chains. They are frequently modified by 9-O-acetylation, and this modification is removed by sialic acid acetylesterases. SIAE appears to encode both lysosomal and cytosolic sialic acid acetylesterase isoforms (LSE and CSE, respectively) (Takematsu et al., 1999 [PubMed 10464298]).[supplied by OMIM
Other Designations
Ysg2 homolog|cytosolic sialic acid 9-O-acetylesterase homolog|sialic acid-specific acetylesterase II
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Interactome
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Disease
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