NT5C3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
NT5C3 transfected lysate
Western Blot
Lane 1: NT5C3 transfected lysate ( 31.57 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NT5C3 full-length
Host
Human
Theoretical MW (kDa)
31.57
Interspecies Antigen Sequence
Mouse (93); Rat (93)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NT5C3 antibody (H00051251-B01) by Western Blots.
SDS-PAGE Gel
NT5C3 transfected lysate
Western Blot
Lane 1: NT5C3 transfected lysate ( 31.57 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NT5C3
Entrez GeneID
51251GeneBank Accession#
BC015856Protein Accession#
AAH15856Gene Name
NT5C3
Gene Alias
MGC27337, MGC87109, MGC87828, P5'N-1, PN-I, PSN1, UMPH, UMPH1, cN-III
Gene Description
5'-nucleotidase, cytosolic III
Gene Ontology
HyperlinkGene Summary
Pyrimidine 5-prime-nucleotidase (P5N; EC 3.1.3.5), also called uridine 5-prime monophosphate hydrolase (UMPH), catalyzes the dephosphorylation of the pyrimidine 5-prime monophosphates UMP and CMP to the corresponding nucleosides. There are 2 isozymes of pyrimidine 5-prime nucleotidase in red blood cells, referred to as type I (UMPH1) and type II (UMPH2; MIM 191720). The 2 enzymes are not separable by electrophoresis in humans but have distinct kinetic properties, and the proteins show no homology.[supplied by OMIM
Other Designations
pyrimidine 5'-nucleotidase|uridine 5' monophosphate hydrolase 1
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