CD274 (Human) Recombinant Protein
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Specification
Product Description
Purified CD274 (AAH74984.1, 18 a.a. - 238 a.a.) human recombinant protein with His-Flag-StrepII tag at N-terminus expressed in human cells.
Transfected Cell Line
Human HEK293T cells
Sequence
AFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNER
Host
Human
Theoretical MW (kDa)
29.59
Interspecies Antigen Sequence
Mouse (69); Rat (69)
Form
Liquid
Preparation Method
Transfection of pSuper-CD274 plasmid into HEK293T cell, and the expressed protein was purified by Strep-Tactin affinity column.
Purification
Strep-Tactin affinity columns
Concentration
≥ 10 ug/ml
Quality Control Testing
SDS-PAGE and Western Blot
SDS-PAGE Gel
Western Blot
Storage Buffer
100 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM EDTA, and 5 mM desthiobiotin.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Enzyme-linked Immunoabsorbent Assay
SDS-PAGE
Protein Interaction
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Gene Info — CD274
Entrez GeneID
29126GeneBank Accession#
BC074984.2Protein Accession#
AAH74984.1Gene Name
CD274
Gene Alias
B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1, PDCD1LG1, PDL1
Gene Description
CD274 molecule
Omim ID
605402Gene Ontology
HyperlinkOther Designations
CD274 antigen|OTTHUMP00000021029|programmed cell death 1 ligand 1
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Interactome
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Pathway
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Disease
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