MRPL18 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MRPL18 full-length
Host
Human
Theoretical MW (kDa)
19.91
Interspecies Antigen Sequence
Mouse (83); Rat (81)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MRPL18 antibody (H00029074-B01) by Western Blots.
SDS-PAGE Gel
MRPL18 transfected lysate.
Western Blot
Lane 1: MRPL18 transfected lysate ( 19.91 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MRPL18
Entrez GeneID
29074GeneBank Accession#
NM_014161.2Protein Accession#
NP_054880.2Gene Name
MRPL18
Gene Alias
HSPC071, L18mt, MRP-L18
Gene Description
mitochondrial ribosomal protein L18
Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L18P ribosomal protein family. Three polymorphic sites exist in this gene, one of which is three nt in length which causes an extra aa near the N-terminus. [provided by RefSeq
Other Designations
39S ribosomal protein L18, mitochondrial|OTTHUMP00000017534
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