GPR77 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
GPR77 transfected lysate.
Western Blot
Lane 1: GPR77 transfected lysate ( 36.1 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GPR77 full-length
Host
Human
Theoretical MW (kDa)
36.1
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GPR77 antibody (H00027202-B01) by Western Blots.
SDS-PAGE Gel
GPR77 transfected lysate.
Western Blot
Lane 1: GPR77 transfected lysate ( 36.1 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GPR77
Entrez GeneID
27202GeneBank Accession#
NM_018485.1Protein Accession#
-Gene Name
GPR77
Gene Alias
C5L2, GPF77
Gene Description
G protein-coupled receptor 77
Omim ID
609949Gene Ontology
HyperlinkGene Summary
The anaphylatoxins C3a (see MIM 120700), C4a (see MIM 120810), and C5a are cationic fragments generated during the complement cascade that participate in host defense. In the case of inappropriate complement activation, anaphylatoxins may be involved in autoimmunity and sepsis. C5L2 is coexpressed with the C5a receptor, C5AR (C5R1; MIM 113995), on polymorphonuclear neutrophils and may modulate C5AR activity (Gerard et al., 2005 [PubMed 16204243]).[supplied by OMIM
Other Designations
G protein-coupled receptor C5L2
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Interactome
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Pathway
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