PSCD4 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PSCD4 transfected lysate.
Western Blot
Lane 1: PSCD4 transfected lysate ( 43.45 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PSCD4 full-length
Host
Human
Theoretical MW (kDa)
43.45
Interspecies Antigen Sequence
Mouse (92); Rat (93)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PSCD4 antibody (H00027128-B01) by Western Blots.
SDS-PAGE Gel
PSCD4 transfected lysate.
Western Blot
Lane 1: PSCD4 transfected lysate ( 43.45 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CYTH4
Entrez GeneID
27128GeneBank Accession#
NM_013385.2Protein Accession#
NP_037517.1Gene Name
CYTH4
Gene Alias
CYT4, DJ63G5.1, PSCD4
Gene Description
cytohesin 4
Omim ID
606514Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. The encoded protein exhibits GEP activity in vitro with both ARF1 and ARF5 but is inactive with ARF6. The structures of this gene and CYTH1 are very similar. [provided by RefSeq
Other Designations
OTTHUMP00000028826|cytohesin-4|pleckstrin homology, Sec7 and coiled-coil domains 4|pleckstrin homology, Sec7 and coiled/coil domains 4
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