POFUT1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
POFUT1 transfected lysate.
Western Blot
Lane 1: POFUT1 transfected lysate ( 22.30 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-POFUT1 full-length
Host
Human
Theoretical MW (kDa)
22.3
Interspecies Antigen Sequence
Mouse (90); Rat (89)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-POFUT1 antibody (H00023509-D01P) by Western Blots.
SDS-PAGE Gel
POFUT1 transfected lysate.
Western Blot
Lane 1: POFUT1 transfected lysate ( 22.30 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — POFUT1
Entrez GeneID
23509GeneBank Accession#
NM_172236Protein Accession#
NP_758436.1Gene Name
POFUT1
Gene Alias
FUT12, KIAA0180, MGC2482, O-FUT, O-Fuc-T, O-FucT-1
Gene Description
protein O-fucosyltransferase 1
Omim ID
607491Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the glycosyltransferase O-Fuc family. This enzyme adds O-fucose through an O-glycosidic linkage to conserved serine or threonine residues in the epidermal growth factor-like repeats of a number of cell surface and secreted proteins. O-fucose glycans are involved in ligand-induced receptor signaling. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
GDP-fucose protein O-fucosyltransferase 1|OTTHUMP00000030583|OTTHUMP00000030584|o-fucosyltransferase protein
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Interactome
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Disease
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