UFL1 (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human UFL1 full-length ORF ( AAH28608.1, 1 a.a. - 509 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MADAWEEIRRLAADFQRAQFAEATQRLSERNCIEIVNKLIAQKQLEVVHTLDGKEYITPAQISKEMRDELHVRGGRVNIVDLQQVINVDLIHIENRIGDIIKSEKHVQLVLGQLIDENYLDRLAEEVNDKLQESGQVTISELCKTYDLPGNFLTQALTQRLGRIISGHIDLDNRGVIFTEAFVARHKARIRGLFSAITRPTAVNSLISKYGFQEQLLYSVLEELVNSGRLRGTVVGGRQDKAVFVPDIYSRTQSTWVDSFFRQNGYLEFDALSRLGIPDAVSYIKKRYKTTQLLFLKAACVGQGLVDQVEASVEEAISSGTWVDIAPLLPTSLSVEDAAILLQQVMRAFSKQASTVVFSDTVVVSEKFINDCTELFRELMHQKAEKEMKNNPVHLITEEDLKQISTLESVSTSKKDKKDERRRKATEGSGSMRGGGGGNAREYKIKKVKKKGRKDDDSDDESQSSHTGKKKPEISFMFQDEIEDFLRKHIQDAPEEFISELAEYLIKQV
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
84
Interspecies Antigen Sequence
Mouse (91); Rat (91)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — UFL1
Entrez GeneID
23376GeneBank Accession#
BC028608.1Protein Accession#
AAH28608.1Gene Name
UFL1
Gene Alias
KIAA0776, Maxer, NLBP, RCAD, RP3-393D12.1
Gene Description
UFM1-specific ligase 1
Gene Ontology
HyperlinkOther Designations
E3 UFM1-protein ligase 1; LZAP-binding protein; Regulator of CDK5RAP3 and DDRGK1; novel LZAP-binding protein
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Interactome
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Publication Reference
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Optimized protocol to detect protein UFMylation in cells and in vitro via immunoblotting.
Junzhi Zhou, Qian Liang, Maogong Dong, Xiaohe Ma, Yaqi Jin, Di Guan, Jiang Liu, Miao Wang, Yu-Sheng Cong.
STAR Protocols 2022 Jan; 3(1):101074.
Application:Catalytic, Human, Recombinant Protein.
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Optimized protocol to detect protein UFMylation in cells and in vitro via immunoblotting.
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