TNIK monoclonal antibody (M03), clone 3D4
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More Files
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Western Blot (Transfected lysate)
Western Blot analysis of TNIK expression in transfected 293T cell line by TNIK monoclonal antibody (M03), clone 3D4.
Lane 1: TNIK transfected lysate(60.3 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged TNIK is 0.3 ng/ml as a capture antibody.
Western Blot detection against Immunogen (37.73 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant TNIK.
Immunogen
TNIK (AAH55427, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MKKVTDYSSSSEESESSEEEEEDGESETHDGTVAVSDIPRLIPTGAPGSNEQYNVGMVGTHGLETSHADSFSGSISREGTLMIRETSGEKKRSGHSDSNGFAGHINLPDL
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (97)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.73 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of TNIK expression in transfected 293T cell line by TNIK monoclonal antibody (M03), clone 3D4.
Lane 1: TNIK transfected lysate(60.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged TNIK is 0.3 ng/ml as a capture antibody.ELISA
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Gene Info — TNIK
Entrez GeneID
23043GeneBank Accession#
BC055427Protein Accession#
AAH55427Gene Name
TNIK
Gene Alias
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Gene Description
TRAF2 and NCK interacting kinase
Omim ID
610005Gene Ontology
HyperlinkGene Summary
Germinal center kinases (GCKs), such as TNIK, are characterized by an N-terminal kinase domain and a C-terminal GCK domain that serves a regulatory function (Fu et al., 1999 [PubMed 10521462]).[supplied by OMIM
Other Designations
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Interactome
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Disease
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Publication Reference
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TNIK INHIBITOR AND THE USE.
Yamada, Tesshi; Shitashige, Miki; Yokota, Koichi; Sawa, Masaaki; Moriyama, Hideki.
United States Patent Application 2010 Jun; US 20100137386A1.
Application：IF, IP, WB, Human, DLD-1, HCT-116, HEK 293, HeLa cells.
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The kinase TNIK is an essential activator of Wnt target genes.
Mahmoudi T, Li VS, Ng SS, Taouatas N, Vries RG, Mohammed S, Heck AJ, Clevers H.
The EMBO Journal 2009 Nov; 28(21):3329.
Application：IHC-P, Human, Mouse, Colon, Intestinal crypt, Small intestine.
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TNIK INHIBITOR AND THE USE.
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