CCT5 monoclonal antibody (M01), clone 4E5-4B1
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More Files
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Western Blot (Cell lysate)
CCT5 monoclonal antibody (M01), clone 4E5-4B1 Western Blot analysis of CCT5 expression in Hela ( Cat # L013V1 ).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to CCT5 on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 1 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CCT5 is approximately 0.3ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to CCT5 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (85.25 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant CCT5.
Immunogen
CCT5 (AAH06543, 1 a.a. ~ 541 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MASMGTLAFDEYGRPFLIIKDQDRKSRLMGLEALKSHIMAAKAVANTMRTSLGPNGLDKMMVDKDGDVTVTNDGATILSMMDVDHQIAKLMVELSKSQDDEIGDGTTGVVVLAGALLEEAEQLLDRGIHPIRIADGYEQAARVAIEHLDKISDSVLVDIKDTEPLIQTAKTTLGSKVVNSCHRQMAEIAVNAVLTVADMERRDVDFELIKVEGKVGGRLEDTKLIKGVIVDKDFSHPQMPKKVEDAKIAILTCPFEPPKPKTKHKLDVTSVEDYKALQKYEKEKFEEMIQQIKETGANLAICQWGFDDEANHLLLQNNLPAVRWVGGPEIELIAIATGGRIVPRFSELTAEKLGFAGLVQEISFGTTKDKMLVIEQCKNSRAVTIFIRGGNKMIIEEAKRSLHDALCVIRNLIRDNRVVYGGGAAEISCALAVSQEADKCPTLEQYAMRAFADALEVIPMALSENSGMNPIQTMTEVRARQVKEMNPALGIDCLHKGTNDMKQQHVIETLIGKKQQISLATQMVRMILKIDDIRKPGESEE
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (96); Rat (96)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (85.25 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
CCT5 monoclonal antibody (M01), clone 4E5-4B1 Western Blot analysis of CCT5 expression in Hela ( Cat # L013V1 ).Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to CCT5 on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 1 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CCT5 is approximately 0.3ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to CCT5 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — CCT5
Entrez GeneID
22948GeneBank Accession#
BC006543Protein Accession#
AAH06543Gene Name
CCT5
Gene Alias
CCT-epsilon, CCTE, KIAA0098, TCP-1-epsilon
Gene Description
chaperonin containing TCP1, subunit 5 (epsilon)
Gene Ontology
HyperlinkGene Summary
This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq
Other Designations
T-complex protein 1, epsilon subunit|chaperonin containing TCP1, epsilon
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Interactome
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Disease
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Publication Reference
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Interaction with the CCT chaperonin complex limits APOBEC3A cytidine deaminase cytotoxicity.
Abby M Green, Rachel A DeWeerd, David R O'Leary, Ava R Hansen, Katharina E Hayer, Katarzyna Kulej, Ariel S Dineen, Julia H Szeto, Benjamin A Garcia, Matthew D Weitzman.
EMBO reports 2021 Sep; 22(9):e52145.
Application：IP, WB-Tr, Human, CEM, HEK 293T, HepaRG, K-562, MDA-MB-231, Ramos, U-2 OS cells.
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TRiC controls transcription resumption after UV damage by regulating Cockayne syndrome protein A.
Pines A, Dijk M, Makowski M, Meulenbroek EM, Vrouwe MG, van der Weegen Y, Baltissen M, French PJ, van Royen ME, Luijsterburg MS, Mullenders LH, Vermeulen M, Vermeulen W, Pannu NS, van Attikum H.
Nature Communications 2018 Mar; 9(1):1040.
Application：WB, Human, VH10-hTert cells.
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Evaluation of formalin-fixed paraffin-embedded tissues in the proteomic analysis of parathyroid glands.
Donadio E, Giusti L, Cetani F, Da Valle Y, Ciregia F, Giannaccini G, Pardi E, Saponaro F, Torregrossa L, Basolo F, Marcocci C, Lucacchini A.
Proteome Science 2011 Jun; 9(1):29.
Application：WB-Ti, Human, Parathyroid adenoma.
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A proteomic approach to study parathyroid glands.
Giusti L, Cetani F, Ciregia F, Da Valle Y, Donadio E, Giannaccini G, Banti C, Pardi E, Saponaro F, Basolo F, Berti P, Miccoli P, Pinchera A, Marcocci C, Lucacchini A.
Molecular BioSystems 2011 Mar; 7(3):687.
Application：WB-Ti, Human, Parathyroid.
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Chaperonin TRiC/CCT participates in replication of hepatitis C virus genome via interaction with the viral NS5B protein.
Inoue Y, Aizaki H, Hara H, Matsuda M, Ando T, Shimoji T, Murakami K, Masaki T, Shoji I, Homma S, Matsuura Y, Miyamura T, Wakita T, Suzuki T.
Virology 2011 Feb; 410(1):38.
Application：WB-Ce, WB-Tr, Human, RCYM1, JFH-1, SGR, Huh-7.5.1 cells.
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Interaction with the CCT chaperonin complex limits APOBEC3A cytidine deaminase cytotoxicity.
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