SIAHBP1 293T Cell Transient Overexpression Lysate(Denatured)

Catalog # H00022827-T01

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Size:100 uL
Price: USD $ 247.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
SDS-PAGE Gel
QC Test

SDS-PAGE Gel

PUF60 transfected lysate.

Western Blot
QC Test

Western Blot

Lane 1: PUF60 transfected lysate ( 59.9 KDa)
Lane 2: Non-transfected lysate.

  • Specification

    Transfected Cell Line

    293T

    Plasmid

    pCMV-SIAHBP1 full-length

    Host

    Human

    Theoretical MW (kDa)

    59.9

    Interspecies Antigen Sequence

    Mouse (97); Rat (97)

    Quality Control Testing

    Transient overexpression cell lysate was tested with Anti-SIAHBP1 antibody (H00022827-B01) by Western Blots.

    SDS-PAGE Gel

    PUF60 transfected lysate.

    Western Blot

    Lane 1: PUF60 transfected lysate ( 59.9 KDa)
    Lane 2: Non-transfected lysate.

    Storage Buffer

    1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

    Storage Instruction

    Store at -80°C. Aliquot to avoid repeated freezing and thawing.

  • Applications

    Western Blot

  • Gene Info — PUF60

    Entrez GeneID

    22827

    GeneBank Accession#

    NM_078480

    Protein Accession#

    NP_510965

    Gene Name

    PUF60

    Gene Alias

    FIR, FLJ31379, RoBPI, SIAHBP1

    Gene Description

    poly-U binding splicing factor 60KDa

    Omim ID

    604819

    Gene Ontology

    Hyperlink

    Gene Summary

    The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene. [provided by RefSeq

    Other Designations

    FBP interacting repressor|Ro ribonucleoprotein binding protein 1|Ro ribonucleoprotein-binding protein 1|fuse-binding protein-interacting repressor|poly-U binding splicing factor PUF60|pyrimidine tract binding splicing factor|siah binding protein 1

  • Interactome
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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