MGAT4B monoclonal antibody (M01), clone 1F4
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More Files
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Western Blot (Tissue lysate)
MGAT4B monoclonal antibody (M01), clone 1F4. Western Blot analysis of MGAT4B expression in human pancreas.
Immunofluorescence
Immunofluorescence of monoclonal antibody to MGAT4B on HeLa cell . [antibody concentration 10 ug/ml]
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant MGAT4B.
Immunogen
MGAT4B (NP_055090, 401 a.a. ~ 500 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
AEVSTSLKTYQHFTLEKAYLREDFFWAFTPAAGDFIRFRFFQPLRLERFFFRSGNIEHPEDKLFNTSVEVLPFDNPQSDKEALQEGRTATLRYPRSPDGY
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (98); Rat (98)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
MGAT4B monoclonal antibody (M01), clone 1F4. Western Blot analysis of MGAT4B expression in human pancreas.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to MGAT4B on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — MGAT4B
Entrez GeneID
11282GeneBank Accession#
NM_014275Protein Accession#
NP_055090Gene Name
MGAT4B
Gene Alias
GNT-IV, GNT-IVB
Gene Description
mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B
Omim ID
604561Gene Ontology
HyperlinkGene Summary
This gene encodes a key glycosyltransferase that regulates the formation of tri- and multiantennary branching structures in the Golgi apparatus. The encoded protein, in addition to the related isoenzyme A, catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc in a beta-1,4 linkage to the Man-alpha-1,3-Man-beta-1,4-GlcNAc arm of R-Man-alpha-1,6(GlcNAc-beta-1,2-Man-alpha-1,3)Man-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1-Asn. The encoded protein may play a role in regulating the availability of serum glycoproteins, oncogenesis, and differentiation. [provided by RefSeq
Other Designations
UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IV|alpha-1,3-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase|aminyltransferase|mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransfera
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Interactome
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Pathway
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Disease
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Publication Reference
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In Situ Proximity Ligation Assay (PLA) Analysis of Protein Complexes Formed Between Golgi-Resident, Glycosylation-Related Transporters and Transferases in Adherent Mammalian Cell Cultures.
Maszczak-Seneczko D, Sosicka P, Olczak T, Olczak M.
Methods in Molecular Biology (Clifton, N.J.) 2016 Sep; 1496:133.
Application：PLA, Human, COS- 7, HeLa , HEK 293T cells.
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In Situ Proximity Ligation Assay (PLA) Analysis of Protein Complexes Formed Between Golgi-Resident, Glycosylation-Related Transporters and Transferases in Adherent Mammalian Cell Cultures.
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