SNF8 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
SNF8 transfected lysate.
Western Blot
Lane 1: SNF8 transfected lysate ( 28.49 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SNF8 full-length
Host
Human
Theoretical MW (kDa)
28.49
Interspecies Antigen Sequence
Mouse (98); Rat (98)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SNF8 antibody (H00011267-B01) by Western Blots.
SDS-PAGE Gel
SNF8 transfected lysate.
Western Blot
Lane 1: SNF8 transfected lysate ( 28.49 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SNF8
Entrez GeneID
11267GeneBank Accession#
NM_007241.2Protein Accession#
NP_009172.2Gene Name
SNF8
Gene Alias
Dot3, EAP30, VPS22
Gene Description
SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae)
Omim ID
610904Gene Ontology
HyperlinkGene Summary
SNF8, VPS25 (MIM 610907), and VPS36 (MIM 610903) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. SNF8, VPS25, and VPS36 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).[supplied by OMIM
Other Designations
EAP30 subunit of ELL complex
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Interactome
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Pathway
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